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KMID : 1123920060200061584
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Korean Journal of Oriental Physiology and Pathology
2006 Volume.20 No. 6 p.1584 ~ p.1592
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Induction of Apoptotic Cell Death by Methanol Extract of Houttuynia cordata Thunb. in A549 Human Lung Carcinoma Cells
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Hong Su-Hyun
Park Cheol
Hong Sang-Hoon
Choi Byung-Tae
Lee Yong-Tae
Park Dong-Il
Choi Yung-Hyun
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Abstract
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Houttuynia cordata Thunb, well known as ’E-Sung-Cho’ in Korea, is traditional medicinal plant generally used in Oriental medicine therapy. We previously reported that the water extract of H. cordata inhibited cell proliferation and induced apoptosis in human breast carcinoma cells. In the present study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of H. cordata (MEHC) in human lung carcinoma A549 cells. It was found that MEHC could inhibit the cell growth in a dose-dependent manner, which was associated with morphological change and apoptotic cell death as determined by formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase cells. Apoptosis of A549 cells by MEHC was also connected with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. MEHC treatment induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant inhibition of poly(ADP-ribose) polymerase (PARP), -catenin and phospholipase (PLC)-1 protein expression. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.
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KEYWORD
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Houttuynia cordata Thunb, apoptosis, A549, Bcl-2, IAPs, caspases
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